c myc peptide C - GenScript peptides polypeptide protein tag derived from the c-myc gene product The Indispensable c-Myc Peptide in Molecular Biology and Research

Myc peptidesequence The c-Myc peptide is a critical tool in molecular biology, primarily recognized for its role in facilitating various laboratory techniques involving the c-Myc proto-oncogene. This gene, a powerful oncogene, is known to be dysregulated in approximately 70% of cancers, highlighting its significance in cellular processesThis gene is a proto-oncogene andencodes a nuclear phosphoproteinthat plays a role in cell cycle progression, apoptosis and cellular transformation.. Understanding the function and applications of the c-Myc peptide is essential for researchers working in fields ranging from cancer biology to protein purification and immunoassays.C-MYC-tag Peptide The c-Myc peptide is essentially a short chain of amino acids that mimics a specific, highly recognized region of the full-length c-Myc protein. This mimicry is key to its utility.

Understanding the c-Myc Protein and its Peptide Equivalent

The c-Myc protein itself is a nuclear phosphoprotein encoded by the c-myc genec-Myc tag peptide (EQKLISEEDL). It acts as a transcription factor, playing a crucial role as a key regulator of cell growth, cell differentiation, and apoptosisMyc-Tag - an overview. Its involvement in these fundamental cellular processes makes it a target of intense research, particularly in the context of cancer development and progression. The c-Myc peptide, often derived from the C-terminal amino acids (410-419) of human c-myc protein, or specifically the 10-residue peptide sequence corresponding to the C-terminal of human c-Myc, serves as a synthetic representation of a critical epitope. This epitope is the specific site that antibodies recognize and bind to.

Applications of the c-Myc Peptide in Research

The primary utility of the c-Myc peptide lies in its application as an affinity tag. The Myc-tag is a polypeptide protein tag derived from the c-myc gene product that can be appended to other proteins using recombinant DNA technologyMyc-tag. This tagging strategy simplifies the labeling and detection of proteins, enabling researchers to more easily track and manipulate them in experiments. The c-Myc tag peptide (EQKLISEEDL) is a common sequence used for this purpose, recognized by specific antibodies such as the 9E10 antibody.A myc tag is apolypeptide protein tag derived from the c-myc gene productthat can be added to a protein using recombinant DNA technology.

One of the most frequent uses of the c-Myc peptide is for displacement of c-Myc-tagged fusion proteins bound to anti-c-Myc antibodies in immunoassays. In techniques like immunoprecipitation or Western blotting, where anti-c-Myc antibodies are used to detect or purify proteins containing the Myc-tag, the c-Myc peptide can act as a competitor. By overwhelming the antibody with the free peptide, researchers can effectively compete out anti-c-Myc antibody binding to the target protein. This is particularly useful for eluting c-Myc-tagged proteins from affinity matrices, such as Thermo Scientific Pierce Anti-c-Myc Agarose, which is an ideal resin for the purification of recombinant c-Myc-tagged proteins. The successful inhibition of antibody binding by the c-Myc peptide ensures that the eluted protein is minimally contaminated by antibody fragments.C-MYC-tag Peptide

Furthermore, the c-Myc peptide is valuable when researchers need to validate antibody specificity.c-Myc Peptideis a useful for displacement of c-Myc-tagged fusion proteins bound to anti-c-Myc antibodies in immunoassays. By using the peptide as a control, they can confirm that the antibody is indeed binding to its intended target epitope on the c-Myc-tagged protein and not to other cellular components.Thec-Myc Peptideis a synthetic peptide with an amino acid sequence that corresponds to the amino acids 410-419 of theC-terminal of humanc-myc. The c-Myc tag peptide is often used as a protein modification to simplify these capture and detection applications.c-Myc Peptide Tag (Previously Covance catalog# PEP-150P)

Commercial Availability and Forms of c-Myc Peptide

The c-Myc peptide is commercially available from various suppliers, often in different forms and quantities to suit specific research needs. You can find it as a lyophilized powder, typically with high purity (>97%)MYC blocking peptide. [Similar Products]. Antibody/Peptide Pairs.c-Myc peptide(MBS9614845) is used for blocking the activity of c-Myc antibody (MBS9600292).. Some vendors offer it as a c-Myc Peptide (TFA), where TFA (trifluoroacetic acid) is used as a counter-ion, which can affect solubility and stabilityMYC blocking peptide. [Similar Products]. Antibody/Peptide Pairs.c-Myc peptide(MBS9614845) is used for blocking the activity of c-Myc antibody (MBS9600292).. In addition to bulk quantities, Pierce c-Myc Peptide 5 mg is a common offering for smaller-scale applications. The availability of these different forms underscores the widespread adoption of the c-Myc peptide in scientific laboratories.c-Myc tag peptide (EQKLISEEDL) Researchers looking to order peptides online will find numerous options for c-Myc peptide from reputable chemical suppliers, including those listed on peptidedirectory platformsc-Myc Peptide Tag (Previously Covance catalog# PEP-150P).

Broader Significance and Future Directions

While the c-Myc peptide is primarily a laboratory reagent, the underlying c-myc gene and its protein product have profound implications in disease. Dysregulation of c-Myc is implicated in various cancers, including B-Cell Lymphomas and lung cancer, where its aberrant expression contributes to malignancy. Research into targeting c-Myc, despite its complex multifaceted function which complicates effective drug targeting, continues to be a significant area of cancer therapy research. Strategies involving novel peptide nuclear delivery systems or other therapeutic approaches aimed at modulating c-Myc activity are under investigation. The c-Myc peptide itself, while not a therapeutic agent, serves as an indispensable tool for understanding these complex biological processes and for developing new diagnostic and therapeutic strategies.Myc-tag is a peptide tag derived from the c-Myc protein. The Myc-tag can be used for many capture and detection applications such as immunoprecipitation, ... The c-Myc peptide is a testament to how understanding the molecular details of key cellular regulators can lead to powerful research toolsThermo Scientific Pierce Anti-c-Myc Agaroseis a high-affinity immunoprecipitation resin ideal for purification of recombinant c-Myc-tagged proteins expressed ....